Endogenously synthesized peptide with an endoplasmic reticulum signal sequence sensitizes antigen processing mutant cells to class I-restricted cell-mediated lysis.

摘要: The HLA-A2-positive human mutant cell line T2 is not lysed by influenza virus-specific HLA-A2-restricted cytotoxic lymphocytes after virus infection. However, lysis does occur when cells are incubated with the antigenic matrix protein-derived peptide M57-68. To examine nature of this defect, were transfected two different plasmids. One plasmid encoded M57-68, and other same preceded an endoplasmic reticulum translocation signal sequence. Mutant expressing M57-68 without sequence susceptible to M57-68-specific T lymphocytes, whereas plus effectively. Lysis parental T1 either was equally effective. These results suggest that defective in transport peptides from cytosol into secretory pathway.