Magnetic selection of transiently transfected cells

摘要: An efficient and rapid method for selecting transiently transfected cells is described. A plasmid encoding a neural cell-specific surface marker co-transfected into mammalian along with the gene of interest. After uptake expression these two plasmids, are immuno-adsorbed to magnetic beads pre-coated antibodies against marker. These immuno-complexes then isolated by means strong magnet. In single round selection, we were able enrich cell population more than 7-fold reporter. specifically selected can now be used either further cultivation or immediate analysis. This has been shown effective on HeLa CV-1 expected give similar results any other transfectable, non-neuronal lines.