Biochemical properties of the outer membrane of Treponema denticola.

摘要: The outer membranes (OMs) from serovars a, b, and c of Treponema denticola, originally isolated periodontal patients, were prepared. Dialysis the OMs against 20 mM MgCl2 yielded aggregable (A) nonaggregable (NA) moieties OMs. absence muramic acid, adenosine triphosphatase, hexokinase, nucleic acid as well electron microscopy indicated that OM preparations homogeneous. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis A NA showed approximately 25 Coomassie brilliant blue R-250 stain-positive bands or 47 silver-stained polypeptides. relative molecular masses ranged between 14 97 kDa. electrophoretic polypeptide profiles shared many similarities among c. However, they exhibited variation in overall pattern, intensity, location stained zones. This was especially true for serovar b. Two-dimensional studies an excess 100 spots with isoelectric points 4.6 to 7.0 14- 97-kDa range. contained simple proteins, glycoproteins, lipoproteins. 4 6, 10 12, 6 glycopeptides two, seven, two lipoprotein c, respectively. 7 9, 11 13 5 four, five, three Lipopolysaccharide detected following removal proteins proteinase K, pronase silver staining sodium gels, lipopolysaccharide by hot phenol extraction. 66- 53-kDa present b while a band mass 45 kDa only Endotoxin-like activity also shown Limulus amebocyte clotting assay chick embryo lethality test. is first report on selected biochemical properties macromolecules known T. denticola. Images