Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: clinical implications.

摘要: PURPOSETo determine the frequency with which prostate-specific antigen (PSA)-positive cells can be detected in peripheral blood of patients prostatic cancer different stages and sensitivities to hormonal therapy.PATIENTS AND METHODSPeripheral from 107 men 27 non-prostate controls was analyzed for PSA mRNA using reverse-transcriptase polymerase chain reaction (RT-PCR) Southern blotting.RESULTSThe lower limit detection one PSA-producing cell diluted into 1 x 10(6) mononuclear cells. The test four 25 (16%) clinically organ-confined (T1-2) disease, three 10 (30%) T3-4 or N+ tumors, 72 (35%) distant metastases. None control samples were positive. An increase positivity observed increasing levels. Within subgroup metastases, six 16 (38%) normal undetectable levels afte...