The Nucleotide Sequences of Tyrosine Transfer RNAs of Escherichia coli. Sequences of the Amber Supressor suIII+ Transfer RNA, the Wild Type suIII- Transfer RNA and Tyrosine Transfer RNAs Species I and II

摘要: The tyrosine tRNAs specified by the E. coli amber suppressor gene su:,, and wild type non-suppressing allele su,, were selectively labelled with 32P in infected transducing phage p80 carrying either of these genes. determination nucleotide sequence SU:,, tRNA is described. This has anticodon CUA. su~, differs from a single GUA where G modified guanylic acid residue. su- cells contain two (I 11). Species I identical to while species I1 nucleotides variable loop region. * normally contains types 11) differing each other region between stem ‘TYCG loop’. There are structural genes for I, gene(s) not been located. SUIII one I. In strains this mutated give which can recognise codon UAG. change first base its anticodon. These results have briefly reported Goodman et al. [l] communication describes detailed three different sequences coli: namely su:,,; sunI (which identical); 11. determinations we used methods developed Sanger [2] Brownlee [3] applicable small amounts high specific activity 32P-labelled tRNA. Quite apart rapidity simplicity methods, their use was dictated technique preparing su;, tRNAs, isolated after infection bacteria phages as + or - allele.