High-performance liquid chromatography tandem mass spectrometry procedure with automated solid phase extraction sample preparation for the quantitative determination of paclitaxel (Taxol®) in human plasma

作者: Cristina Sottani , Claudio Minoia , Maurizio D'Incalci , Massimiliano Paganini , Massimo Zucchetti

DOI: 10.1002/(SICI)1097-0231(19980314)12:5<251::AID-RCM145>3.0.CO;2-Z

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摘要: A sensitive, specific, accurate and reproducible analytical method was developed validated for the quantitation of anticancer agent paclitaxel in human plasma. This procedure is based on high performance liquid chromatography/ion spray-tandem mass spectrometry. methodology highly specific because a MS/MS technique (multiple reactant-ion monitoring, MRM) used both its internal standard. The use fully automated solid phase extraction procedure, using CN Sep-pak cartridge, to improve detection limit quantification plasma samples, evaluated. involves addition methyl-paclitaxel as standard (i.s.). retention times I.S. were 2.8 4.0 min., respectively. assay linear over range 5 500 ng/mL, with ng/mL having coefficient variation (c.v.) < 10%. Standard calibration curves, performed three different days, had correlation coefficients always greater than 0.998. intra inter-day precision within 12%, accuracy included 102-110%. Paclitaxel recovery assessed at 15,250 determined be 85%. applicable clinical pharmacokinetic studies.

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