作者: Joan Camunas-Soler , Marco Ribezzi-Crivellari , Felix Ritort
DOI: 10.1146/ANNUREV-BIOPHYS-062215-011158
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摘要: We review the current knowledge on use of single-molecule force spectroscopy techniques to extrapolate elastic properties nucleic acids. emphasize lesser-known single-stranded DNA. discuss importance accurately determining response in pulling experiments, and we simplest models used rationalize experimental data as well approaches pull Applications investigate DNA conformational transitions secondary structure formation are also highlighted. Finally, provide an overview effects salt temperature briefly contour length sequence dependence.