Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera.

摘要: Developmental variability was introduced into Withania somnifera using genetic transformation by Agrobacterium rhizogenes, with the aim of changing withasteroid production. Inoculation W. A. rhizogenes strains LBA 9402 and A4 produced typical transformed root lines, callus rooty lines simultaneous dedifferentiation redifferentiation. These morphologically distinct varied in T-DNA content, growth rates, accumulation. All morphology contained TL T-DNA, 90% them carried TR irrespective strain used for infection. Accumulation withaferin A maximum (0.44% dry weight) line WSKHRL-1. This is first detection roots somnifera. induced both TR-DNAs. In contrast, 50% rooty-callus obtained only T-DNA. accumulated withanolide D. The callusing lacked genes, while all DNA. Four these (0.15–0.21% D (0.08–0.11% weight), they grew faster than lines. report presence withasteroids undifferentiated cultures