Disease-duration based comparison of subsets of immune cells in SARS CoV-2 infected patients presenting with mild or severe symptoms identifies prognostic markers for severity.

摘要: INTRODUCTION: Infection with SARS-CoV-2 leads to a spectrum of symptoms. Understanding the basis for severity remains crucial better management and therapy development. So far, older age, associated-comorbidities, IL-6 have been associated severity/mortality. MATERIALS AND METHODOLOGY: As primary step, we analyzed frequency functional profile innate immune cells (NK cells/dendritic cells/monocytes) adaptive immunity-driving lymphocytes (B cells/T cells/follicular T helper cells) by flow cytometry. Sixty cases SARS CoV-2 infection (25 severe, 35 mild) ten healthy subjects without IgG were included. Disease-duration based analysis was explored early events differentiating two disease forms. Neutralizing antibody titers determined PRNT. RESULTS CONCLUSION: Disease found be impaired maturation mDCs hyperactivation NK, follicular cells, CD8 cells. Lower IL-21 receptor expression on memory B indicated an imbalance in IL-21/IL-21 R ratio. BCMA positive plasmablast severe did suggest probable absence long-term humoral immunity. Multivariate revealed progressive association PD-1+CD4 PRNT50 titers. Thus, addition identifying prognostic markers severity, our study emphasizes definite need in-depth viral antigen-specific analyses larger patient cohort multiple sampling.