Uterine and vaginal insemination optimised in brushtail possums (Trichosurus vulpecula) superovulated with pregnant mare serum gonadotrophin and porcine luteinising hormone

摘要: Artificial insemination of brushtail possums (Trichosurus vulpecula) is being developed as an assisted breeding model for endangered marsupials, well a bioassay testing fertility control vaccines to manage overabundant populations. Procedures were optimised in animals superovulated with pregnant mare serum gonadotrophin (PMSG) and porcine luteinising hormone (pLH). Of three intervals examined, yields maximal following uterine at 27–29.5 h after pLH treatment (four eggs, two embryos per female). Compared no insemination, uterine-inseminated ovulated 30–36 rather than 28–34 treatment. For the vaginal route, 10–13 (six seven four female) five other intervals, when using acclimatised females during autumn season. This protocol was suitable April–June influenced by housing location animals, presence active corpus luteum PMSG batch, but not factors (year trial, Freund’s adjuvant treatment, changes bodyweight, dose kg–1). Embryos eight- 16-cell or unilaminar blastocyst stage respectively. With timing artificial optimised, new methods synchronise induce oestrus ovulation are required achieve year-round birth offspring.