The Transcription Factor Evi-1 Is Overexpressed, Promotes Proliferation, and Is Prognostically Unfavorable in Infratentorial Ependymomas

摘要: Purpose: Ependymomas are glial tumors of presumably radial origin that share morphologic similarities with ependymal cells. The molecular genetics ependymomas supratentorial, infratentorial, and spinal location is heterogeneous. We aimed at identifying pathways operative in the development infratentorial ependymomas. Experimental Design: To do so, gene expression profiles tumor cells laser microdissected from ( n = 15) were compared nonneoplastic autopsy tissue 7). Results: Among 31 genes significantly overexpressed (>5-fold) ependymomas, transcription factor EVI1 (ecotropic viral integration site 1) showed highest overexpression (35-fold). Evi-1 protein could be confirmed formalin-fixed, paraffin-embedded samples 26 28 but only 7 47 nonependymal P MDS1/EVI1 fusion transcripts detectable 17 correlated MGMT (O6-methylguanine-DNA-methyltransferase) promoter hypermethylation -specific siRNAs resulted significant growth inhibition [48 hours: 87% ± 2% 74% 10% as control (mean SD; further validated an independent cohort 39 supratentorial on basis mRNA profiling. Although status had no prognostic impact, high was associated shorter overall survival progression-free survival. Conclusions: conclude, promotes proliferation cells, prognostically unfavorable. Clin Cancer Res; 17(11); 3631–7. ©2011 AACR .