Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia).

摘要: Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization filter hybridization. They all localized to centromeric regions autosomes categorized into major satellite DNA, type I minor, II minor sequences. The sequence also hybridized interspersedly in non-centromeric regions. sequence, which consisted 30 bp elements, was organized tandem arrays constituted majority heterochromatin. with B chromosomes different patterns, suggesting that A. peninsulae derived A three amplified independently on each chromosome. are present genomes other seven Apodemus species. In contrast, had a low homology only few These results suggest base substitution after divergence genus common ancestor might have species-specific origin.