The effects of pH and semiquinone formation on the oxidation-reduction potentials of flavin mononucleotide. A reappraisal.
作者： Stephen G. Mayhew
DOI: 10.1046/J.1432-1327.1999.00767.X
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摘要: Calculation shows that there is poor agreement between frequently cited values for the midpoint redox potentials of two one-electron steps in reduction flavin mononucleotide and equations lines relate these to pH use published pKa three states [Draper, R. & Ingraham, L.L. (1969) Arch. Biochem. Biophys. 125, 802-808]. Equilibrium data first step obtained by pulse radiolysis [Anderson, R.F. (1983) Biochim. Acta 722, 158-162] show much closer with theory lead semiquinone formation constant are close those derived from measurements radical concentration using ESR spectroscopy. It concluded second method more reliable. The at 7.0 20 degrees C calculated be -0.207 V overall two-electron (Em), -0.313 oxidized (E2) -0.101 hydroquinone (E1). Information provided allow calculation other physiological range.
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