Enhanced bile canaliculi formation enabling direct recovery of biliary metabolites of hepatocytes in 3D collagen gel microcavities
作者： Hitoshi Matsui , Shoji Takeuchi , Tomoharu Osada , Teruo Fujii , Yasuyuki Sakai
DOI: 10.1039/C2LC40046D
关键词:
摘要: Analysis of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. In this paper, we present a hepatocyte culture configuration that enables the direct recovery bile acid accumulates in canaliculi by embedding hepatocytes 3D micropatterned collagen gel substrate. We investigated formation embedded circular microcavities various sizes made from gel. Image analyses using fluorescently labeled revealed area microcavity 60 or 80 μm diameter was enlarged when compared with other sized those cultured conventional sandwich cultures. successfully recovered glass capillary quantified amount recovered. Using our approach, metabolites, cultures enhanced excretion geometrically canaliculi, may enable accurate screening drug–drug interactions against transporters.
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