The Capsicum annuum class IV chitinase ChitIV interacts with receptor-like cytoplasmic protein kinase PIK1 to accelerate PIK1-triggered cell death and defence responses

摘要: The pepper receptor-like cytoplasmic protein kinase, CaPIK1, which mediates signalling of plant cell death and defence responses was previously identified. Here, the identification a class IV chitinase, CaChitIV, from plants (Capsicum annuum), interacts with CaPIK1 promotes CaPIK1-triggered responses, is reported. CaChitIV contains signal peptide, chitin-binding domain, glycol hydrolase domain. expression up-regulated by Xanthomonas campestris pv. vesicatoria (Xcv) infection. Notably, avirulent Xcv infection rapidly induced in leaves. Bimolecular fluorescence complementation co-immunoprecipitation revealed that planta, CaPIK1-CaChitIV complex localized mainly cytoplasm plasma membrane. also endoplasmic reticulum. Transient co-expression enhanced response reactive oxygen species (ROS) nitric oxide (NO) bursts. Co-silencing both conferred susceptibility to infection, accompanied reduced induction response, ROS NO bursts, genes. Ectopic Arabidopsis basal resistance Hyaloperonospora arabidopsidis Together, results suggest positively regulates through its interaction CaPIK1.