Two monoclonal antibodies that discriminate between allelic variants of human low density lipoprotein.
作者： PETER SCHLAPFER , THERESE NYDEGGER , ESTHER BÜTLER-BRUNNER , JEAN-JACQUES MORGENTHALER , RENÉ BÜTLER
关键词:
摘要: Human low density lipoprotein shows a genetic polymorphism, the so-called Ag-system. It consists of 5 pairs allelic epitopes, x/y, a1/d, c/g, t/z, and h/i, which are localized on apolipoprotein B. We have generated large number monoclonal antibodies against lipoprotein. Two them, D2E1 H11G3, recognize epitopes related to this polymorphism. Direct ELISA inhibition experiments with different lipoproteins known phenotype showed that is directed epitope c H11G3 d. The two were used for characterization in sera from blood donors results compared those obtained by passive hemagglutination using human allotypic antisera. Sera homo- or heterozygous (which display relevant epitope) could be distinguished homozygous lack described.
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