Relative amounts and molecular forms of NESP55 in various bovine tissues

摘要: NESP55 (neuroendocrine secretory protein with Mr 55,000) comprises a novel chromogranin-like protein, which is paternally imprinted at the genomic level. We used antisera raised against GAIPIRRH, peptide present C-terminus of this and TC-14, located in N-terminal half NESP55. Radioimmunoassay, gel-filtration chromatography immunoblotting were to determine levels molecular forms different bovine organs. The tissues highest GAIPIRRH immunoreactivity were, decreasing order: adrenal medulla, anterior pituitary, posterior various brain regions, intestine. degree proteolytic processing revealed differences among analyzed. lowest was detected pituitary where only peak corresponding intact precursor present. This also true for cerebrospinal fluid (CSF). In intestine, free predominant form. GAIPIRRH-IR, as CSF, serum mainly an precursor. A relatively high concentration GAIPIRRH-IR found kidney probably due endocytotic re-uptake molecule from tubuli after filtration glomeruli. study consistent concept that NESP55, like other chromogranins, becomes proteolytically processed. function new therefore, might be represent biologically active peptides.