Immune Responses to Allergens in Atopic Disease: Considerations for Bioinformatics

摘要: Allergies are mediated by several immunological mechanisms that make different contributions in patients. Measurements of Th2 and regulatory T cell responses poorly standardized highly influenced culture conditions allergen preparations. There are, however, purified allergens new techniques can facilitate direct ex vivo measurements for use. IgE antibodies be accurately reproducibly measured using pure represent the important specificities arrays used to identify cross-reactivities, hence, reduce false positives. binding most is dominated a few components usually bind with same pattern patients, although there exceptions now recognized provide information. also underestimation antibody titers due underrepresentation components. Furthermore, should analysed preferably as continuous variables or if cut offs thresholds reflect high probability substantive atopy these differ allergens. The value has historically been indicate reaction sensitivity, 0.35 IU/mL, historic sensitivity trivial amount many but significant others. Positive skin test readily elicited subjects trivially low no history allergic disease thus poor measure atopy. type immune allergens, shown IgG subclass and, from limited data, memory phenotypes cytokine profiles. much considerations applied improve design analysis bioinformatic studies.