Cyclosporine induces different responses in human epithelial, endothelial and fibroblast cell cultures.

摘要: Cyclosporine induces different responses in human epithelial, endothelial and fibroblast cell cultures. Background Nephrotoxicity, accelerated atherosclerosis, graft vascular disease are common complications of cyclosporine long-term treatment characterized by a wide disruption organ architecture with increased interstitial areas accumulation extracellular matrix (ECM). How these changes is not clear, but it conceivable that they the sum induced at level. Methods We studied effects on (HEC), epithelial (HK-2), (MRC5) cells. Cell proliferation was evaluated counting, apoptosis collagen production enzyme-linked immunosorbent assay, nitric oxide measuring concentration nitrite/nitrate supernatant. (α1)I (α2)IV collagen, metalloprotease-9 (MMP9), tissue inhibitors metalloprotease-1 (TIMP-1) mRNA levels were measured reverse transcription-polymerase chain reaction. Proteolytic activity zymography. Results showed marked antiproliferative proapoptotic effect Fibroblast growth affected cyclosporine. Nitric up-regulated cells fibroblasts synthesis cyclosporine-treated cells, respectively. TIMP-1 fibroblasts. Conclusions Our results demonstrate exhibits an This associated induction probably via up-regulation ECM increasing reducing its degradation up-regulating expression conclude affects types differently result multiple