Expression of Genes Involved in Porphyrin Biosynthesis Pathway in the Human Renal Cell Carcinoma.
作者： Hugo Nóbrega da Rocha Filho , Evelin Caroline da Silva , Flávia R. O. Silva , Lilia Coronato Courrol , Carlos Henrique de Mesquita
DOI: 10.1007/S10895-015-1626-X
关键词:
摘要: Renal cell carcinoma (RCC) remains one of the greatest challenges urological oncology and is third leading cause death in genitourinary cancers. Surgery may be curative when patients present with localized disease. Our previous results demonstrated autofluorescence blood PpIX primary RCC mouse model an increase fluorescence intensity as a function growth subcutaneous tumor mass. In another work, nice correlation between mass tissue was found. The aim this study to evaluate expression profile porphyrin biosynthesis pathway-related genes human kidney cells. We used two lines, normal (HK2) malignant (Caki-1). Endogenous 5-aminolevolinic acid (ALA) induced protoporphyrin IX (PpIX) HK2 Caki-1 cells were analyzed by spectroscopy. Real-time quantitative polymerase chain reaction (qRT-PCR) measure mRNA those genes. Emission spectra obtained exciting samples at 405 nm. For ALA untreated maximum detected 635 mean peak area emission both types increased linearly number. Besides, basal levels each concentration significantly lower than samples. ALA-treated shows nm shoulder 700 Analysis ratio showed that was, on average, 26 times greater healthy qRT-PCR revealed cells, PEPT gene up-regulated FECH HO-1 down regulated comparison conclusion, our demonstrate preferential accumulation ALA-induced also indicate PEPT1, are major contributors accumulation.
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