The use of fluorescein diacetate to assess embryo viability in the mouse.

摘要: Preimplantation mouse embryos that were exposed to fluorescein diacetate (FDA) accumulated intracellular and fluoresced brightly under ultraviolet (u.v.) light. The rate at which was lost from the cells measured 37, 28 4 degrees C decreased as storage temperature decreased. increased FDA concentration until a maximum attained. ability accumulate could be removed by heating 56 for 30 min or damaging cell membrane. Cells grown inadequate culture conditions fluorescein. Exposure of 2-cell u.v. light did not alter blastocyst formation in vitro, exposure blastocysts implantation post-implantation development vivo.