Role of planktonic and sessile extracellular metabolic byproducts on Pseudomonas aeruginosa and Escherichia coli intra and interspecies relationships

摘要: Bacterial species are found primarily as residents of complex surface-associated communities, known biofilms. Although these structures prevail in nature, bacteria still exist planktonic lifestyle and differ from those morphology, physiology, metabolism. This study aimed to investigate the influence physiological states Pseudomonas aeruginosa Escherichia coli cell-to-cell interactions. Filtered supernatants obtained under biofilm cultures each single were supplemented with tryptic soy broth (TSB) used growth media (conditioned media) sessile both single- two-species cultures. Planktonic bacterial was examined through OD640 measurement. One-day-old biofilms evaluated terms biomass (CV), respiratory activity (XTT), CFU number. Conditioned either or mode life triggered a synergistic effect on growth, mainly for E. growing P. supernatants. Biofilms grown presence biofilms-derived metabolites presented less mass activity. These events highlight that, when developed biofilm, release signals able prejudice binary others their own species. However, products released by counterparts did not impair coli, living cultures, removed un-beneficial compounds which promoted all Our findings revealed that inter intraspecies behaviors depend involved adopted life.