SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives.
作者： Milutin Stepić , Marijana Petković , Maja D. Nešić , Tanja Dučić , Xinyue Liang
DOI: 10.1016/J.IJBIOMAC.2020.10.141
关键词:
摘要: Abstract Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, diameter particles were determined, cytotoxicity against three cell lines tested. Biochemical changes in HeLa cells analyzed synchrotron radiation (SR) FTIR spectro-microscopy combined principal component analysis (PCA). most significant occur treated LEV-C60 correspond to protein region, i.e. Amide I band, structure lipid bodies membrane fluidity evident. highest was also induced LEV-C60. In cells, could not be strictly associated biochemical lipids, proteins nucleic acids, but these findings contribution mechanism C60-based nanoparticles cellular biomolecules. conclusion, LEV, PUL, CHL, CHP enhanced potential used as target delivery system ability induce specific intracellular
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