Comparative Studies of Vertebrate Immunoglobulins

摘要: IgG and IgM were isolated from 10 different species (human, monkey, horse, dog, cow, sheep, rabbit, chicken, rat guinea pig) by a combination of starch block electrophoresis Sephadex G-200 gel filtration. The patterns heavy light chains studied in urea at pH 3.0 after reduction alkylation. With the exception chicken 7S immunoglobulin, electrophoretic remarkably similar. However, when preparations these compared, marked differences mobility µ seen. immunologic cross-reactions proteins same quantitative complement fixation with three rabbit antisera specific for human γ chains. nonprimate cross-reacted weakly (5 to 30%) compared which strongly (30 55%). In accordance studies, protein was uniquely other fractions did not cross-react all various antihuman chain sera while showed good cross-reactivity.