In vivo clonal analysis reveals lineage-restricted progenitor characteristics in mammalian kidney development, maintenance, and regeneration

摘要: The mechanism and magnitude by which the mammalian kidney generates maintains its proximal tubules, distal collecting ducts remain controversial. Here, we use long-term in vivo genetic lineage tracing clonal analysis of individual cells from kidneys undergoing development, maintenance, regeneration. We show that adult undergoes continuous tubulogenesis via expansions fate-restricted clones. Kidneys recovering damage undergo through clones with segment-specific borders, renal spheres developing vitro maintain distinct, fates. Analysis mice derived transfer color-marked embryonic stem (ESCs) into uncolored blastocysts demonstrates nephrons are polyclonal, singly fated Finally, Wnt-responsive precursors, their tubules segment specific. Collectively, these analyses demonstrate precursors functioning as unipotent progenitors continuously self-preserve mouse throughout life.