Design-Based Stereology for Evaluation of Histological Parameters

摘要: Valid quantification of organ volume and total cell numbers are crucial parameters for morphometric studies. The number a specific type cannot be simply deduced from the its profiles found in thin tissue sections, as this parameter also depends on volume, orientation well atrophy. Design-based stereology has become method choice unbiased, reproducible quantification. Steps described protocol include transcardial perfusion mice, postfixation, cryoprotection region interest (ROI), followed by preparation systematically randomly sampled series thick sections through entire ROI. Furthermore, it is how to perform immuno-histochemical staining such cryo-sections, providing guidance ROI generation unbiased virtual counting spaces, steps work with these spaces obtain an estimate numbers.