Optimization of GFP Fluorescence Preservation by a Modified uDISCO Clearing Protocol.

摘要: Tissue optical clearing techniques provide alternative approaches for imaging large-volume specimens. uDISCO, an organic-solvent-based method, stands out from the enormous array of available methods by achieving whole-brain with high transparency, size reduction and fluorescence preservation. In this study, we aimed to modify uDISCO protocol achieve better preservation thereby further improve its quality. First, determined optimal pH value optimized termed "a-uDISCO" (alkaline pH-based uDISCO). Then, compared between a-uDISCO uDISCO. addition, validated performance method according several parameters, including tissue changes, maintenance cell morphology. Finally, demonstrated that enabled high-quality brain-wide visualization neuronal structures. This potentially provides a high-throughput samples low-level protein expression or archiving repetitive revisiting rare samples.