Estrogenic Alkylphenols Induce Cell Death by Inhibiting Testis Endoplasmic Reticulum Ca2+ Pumps

摘要: Industrial alkylphenols in the environment may act as “xenoestrogens” to disrupt testicular development and decrease male fertility. Amongst possible targets for these compounds are Sertoli cells, which nurture developing sperm cells. We demonstrate that SERCA 2 3 Ca2+ pumps relatively abundant rat testis microsomal membranes, also Sertoli, myoid, TM4 cells (a cell line). A number of estrogenic such nonylphenol, octylphenol, bisphenol A, butylated hydroxytoluene all inhibit ATPase low micromolar concentration range. These agents mobilize intracellular intact a manner consistent with inhibition ER pumps. Alkylphenols dramatically viability an effect is reversed by either caspase inhibitor or BAPTA, therefore Ca2+-dependent death via apoptosis. postulate inhibiting pumps, thus disturbing homeostasis.