Arachidonic acid-dependent carbon-eight volatile synthesis from wounded liverwort (Marchantia polymorpha)

摘要: Eight-carbon (C8) volatiles, such as 1-octen-3-ol, octan-3-one, and octan-3-ol, are ubiquitously found among fungi bryophytes. In this study, it was that the thalli of common liverwort Marchantia polymorpha, a model plant species, emitted high amounts C8 volatiles mainly consisting (R)-1-octen-3-ol octan-3-one upon mechanical wounding. The induction emission took place within 40min. intact thalli, 1-octen-3-yl acetate predominant volatile while tissue disruption resulted in conversion to 1-octen-3-ol. This carried out by an esterase showing stereospecificity (R)-1-octen-3-yl acetate. From transgenic line M. polymorpha (des6(KO)) lacking arachidonic acid eicosapentaenoic acid, formation only minimally observed, which indicated and/or acids were essential form polymorpha. When des6(KO) exposed vapor they absorbed alcohol converted into octan-3-one. Therefore, implied 1-octen-3-ol primary product formed from further metabolism involving acetylation oxidoreduction occurred diversify products. Octan-3-one completely disrupted most abundant partially thalli. is assumed remaining tissues involved partly promoted addition NAD(P)H tissues, suggesting NAD(P)H-dependent oxidoreductase conversion.