Human airway ciliary dynamics

摘要: Airway cilia depend on precise changes in shape to transport the mucus gel overlying mucosal surfaces. The ciliary motion can be recorded several planes using video microscopy. However, are densely packed, and automated computerized systems not available convert these into forms that useful for testing theoretical models of function. We developed a system converting planar motions by microscopy an empirical quantitative model, which is easy use validating mathematical models, or examining function, e.g., primary dyskinesia (PCD). we allows manipulation model cilium superimposed over beating cilia. Data were analyzed determine shear angles velocity vectors points along cilium. Extracted waveforms used construct composite waveform, could as standard. Variability was measured mean difference position individual shapes end-recovery, end-effective, fastest moving effective recovery with (± SE) differences 0.31(0.04), 0.25(0.06), 0.50(0.12), 0.50(0.10), μm, respectively. In contrast, same measures three different PCD had values far outside this range.