Binding of Affinity Cross-Linked Oligomers of IgG to Cells Bearing Fc Receptors

摘要: The binding of covalently cross-linked oligomers rabbit IgG antibodies to tumor cells resembling macrophages and lymphocytes normal spleen has been measured. With all trimeric binds with greater affinity than does the dimer, which in turn more tightly monomer. However, as increase size, individual monomeric subunits bind decreasing energy. macrophage line, P388D 1 , lymphocyte AKTB-1, but differences affinities are not great, differing by at most a factor 5 equilibrium constant. Normal same concentration range cells. kinetics do occur single first or second order reactions suggest multistage mechanism for oligomer binding. presence large concentrations tends weaken increases exchange rate bound from Since plasma extracellular fluid also contain IgG, it is suggested that many immune complexes will weakly types examined here rapidly external medium.