Harnessing the Rhizosphere of the Halophyte Grass Aeluropus littoralis for Halophilic Plant-Growth-Promoting Fungi and Evaluation of Their Biostimulant Activities

摘要: Hydroponic systems have gained interest and are increasingly used in hot dry desert areas. Numbers of benefits offered by hydroponic such as the ability to save water, enhance nutrients use efficiency, easy environmental control, prevention soil-borne diseases. However, high consumption chemical fertilizers for nutrient solution sensitivity closed salinity issues that need solutions. Thus, main goal our research activities is isolate plant growth promoting fungi order develop sustainable systems. We working on isolating testing possibility incorporate cell-free filtrate (CFF) (PGPF) composition solution. In this work, we isolated six strains PGPF from rhizosphere halophyte grass Aeluropus littoralis. Phylogenetic analyses DNA sequences amplified ITS1 ITS4 primers identified as: Byssochlamys spectabilis, Chaetomium globosum, Cephalotheca foveolata, Penicillium melinii, Alternaria tenuissima, Nigrospora chinensis. The vigor tobacco seedlings was criteria evaluate biostimulant activity these adding either their mycelia (DE: direct effect) or filtrates (CFF: indirect plant-growth media. best significant stimulation obtained with plants treated B. spectabilis. only CFFs spectabilis (A5.1) melinii (A8) when added at a dilution factor 1/50 half-strength nutritive (0.5NS) resulted improvement all assessed parameters. Indeed, A5.1CFF A8CFF 0.5NS induced better increase biomass production compared NS alone. All produced indole acetic acid CFFs, which could be one key factors explaining activities. Furthermore, genes involved nitrogen-metabolism (NR1 NRT1), auxin biosynthesis (Tryp1 YUCCA6-like), brassinosteroid (DET2 DWF4) were shown roots leaves following treatment CFFs. This work opens up prospect study deep PGPFs applications decrease requirement growing