Recombination-mediated PCR-directed plasmid construction in vivo in yeast

作者: Oldenburg Kevin R , Kham T Vo , Susan Michaelis , Chris Paddon

DOI: 10.1093/NAR/25.2.451

关键词:

摘要: … We have extended the technique of PCR-directed recombination in Saccharomyces … homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous …

参考文章(8)
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
Mingfu Ling, Frank Merante, Brian H. Robinson, A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction Nucleic Acids Research. ,vol. 23, pp. 4924- 4925 ,(1995) , 10.1093/NAR/23.23.4924
Palaniyandi Manivasakam, Shane C. Weber, John McElver, Robert H. Schiestl, Micro-homology mediated PCR targeting in Saccharomyces cerevisiae Nucleic Acids Research. ,vol. 23, pp. 2799- 2800 ,(1995) , 10.1093/NAR/23.14.2799
Hong Ma, Sam Kunes, Peter J. Schatz, David Botstein, Plasmid construction by homologous recombination in yeast Gene. ,vol. 58, pp. 201- 216 ,(1987) , 10.1016/0378-1119(87)90376-3
A. Baudin, O. Ozier-Kalogeropoulos, A. Denouel, F. Lacroute, C. Cullin, A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae Nucleic Acids Research. ,vol. 21, pp. 3329- 3330 ,(1993) , 10.1093/NAR/21.14.3329
Andreas Crameri, Erik A. Whitehorn, Emily Tate, Willem P.C. Stemmer, Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnology. ,vol. 14, pp. 315- 319 ,(1996) , 10.1038/NBT0396-315
Chris Paddon, Diego Loayza, Luca Vangelista, Roberto Solari, Susan Michaelis, Analysis of the localization of STE6/CFTR chimeras in a Saccharomyces cerevisiae model for the cystic fibrosis defect CFTRΔF508 Molecular Microbiology. ,vol. 19, pp. 1007- 1017 ,(1996) , 10.1046/J.1365-2958.1996.444973.X