p53 Mutations occur in aggressive breast cancer

摘要: Using a polymerase chain reaction-single strand conformation polymorphism approach we analyzed 96 human primary breast tumors for the presence of mutations in exons 2, 5, 6, 7, 8, and 9 p53 gene. These have been shown to comprise highly conserved sequences portion including 5 through is believed be target over 90% acquired cancer. Eighteen (18.7%) tested showed reproducibly variant band indicative mutation. Most (15 tumors) were single nucleotide substitutions G:C A:T transitions prevalent (6 tumors), T:A transversions came next (4 guanines always on nontranscribed strand. Concomitant loss wild type allele mutation other copy was observed only 3 18 mutated cases; this consistent with heterogeneous cellular composition tumors. Furthermore correlated estrogen and/or progesterone receptor negative tumors, thus indicating their relationships aggressive No association could DNA amplification events these