Differential anti-inflammatory and anti-oxidative effects of dexamethasone and N-acetylcysteine in endotoxin-induced lung inflammation

摘要: Inhalation of bacterial endotoxin induces an acute inflammation in the lower respiratory tract. In this study, anti-inflammatory effects anti-oxidant N-acetylcysteine (NAC) and glucocorticoid dexamethasone were investigated mice exposed to aerosolized (lipopolysaccharide (LPS)). Powerful reduction neutrophils bronchoalveolar lavage fluid (BALF) was obtained by a single i.p. injection (10 mg/kg), whereas treatment with NAC only resulted when administered at high dose (500 mg/kg). Measurement cytokine chemokine expression lung tissue revealed significant decrease tumour necrosis factor-alpha, IL-1alpha, IL-1beta IL-6, IL- 12p40, MIP-1alpha mRNA where treated but not NAC. Analysis oxidative burst demonstrated remarkable oxygen radicals BALF after dexamethasone, effect significantly different from that untreated animals. conclusion, exerted both anti-oxidative airway inflammation, probably blocking early events inflammatory cascade. contrast, weak response no inhibition proinflammatory cytokines or neutrophils. These results demonstrate dramatic differences efficiency also indicate two drugs have actions. Combined additive action synergy observed.