Allelic deletion mapping on chromosome 5 in human carcinomas.

摘要: We analysed allelic deletions on chromosome 5 in microdissected human non-small cell lung cancers. Thirty-four primary squamous carcinomas, 15 adenocarcinomas and five regional lymph node metastases were investigated for loss of heterozygosity (LOH) chromosomal region 5p15-q21. The sites included the APC tumor suppressor gene at 5q21, polymorphic microsatellite markers putative locus del-27, that was assigned to 5p13-12 by fluorescence situ hybridization (FISH) analysis. Allelic encompassed larger genomic regions more often carcinomas than adenocarcinomas. del-27 amd identified as two distinct with highest LOH frequencies within In 73% 70% locus. loci occurred 38% informative cases. deletion also detected metastases. results suggest involvement least genes tumorigenesis.