Construction and analysis of function of G protein-coupled receptor-G protein fusion proteins.

摘要: Publisher Summary In recent times, fusion proteins in which the N terminus of a G protein α subunit is attached directly to C-terminal tail protein-coupled receptor (GPCR) have become popular constructs analysis ligand regulation activation. Although they are clearly unnatural entities, number groups commented on and reviewed many benefits unique characteristics such proteins. all cases reported date, agonist-stimulated GTPase activity has an enzyme that behaves accordance with formalisms derived by Michaelis Menten. Therefore, simple kinetic stimulated provides quantitative means features as distinct efficacy effects point mutations within either GPCR or protein. between GPCRs subunits provided address interactions these manner would not been easy achieve coexpression individual polypeptides.