Detection of a Tadalafil Analogue as an Adulterant in a Dietary Supplement for Erectile Dysfunction

摘要: Introduction. Several cases of adulteration dietary supplements with tadalafil, sildenafil, and vardenafil, or their unapproved analogues have been reported worldwide. Mainly, the presence latter represents a serious health risk to consumers as efficacy toxic effects not assessed may result in unpredictable adverse effects. Aim. To investigate suspected synthetic phosphodiesterase type 5 (PDE-5) inhibitors supplement marketed Argentina for treatment erectile dysfunction (ED). Methods. The content capsules (sample A) was analyzed by thin layer chromatog- raphy (TLC) high-performance liquid chromatography (HPLC) diode-array detection. From organic extract sample A, major compound purified column (CC). isolated identified proton nuclear magnetic resonance (1H NMR) carbon NMR (13C NMR), heteronuclear single quantum coherence, distortionless enhancement polarization transfer (DEPT 135), electrospray ionization mass spectrometry, ultraviolet, infrared (Fourier transform spectroscopy) spectroscopy. Main Outcome Measure. Proof herbal products PDE-5 inhibitors. Results. By TLC HPLC analysis, detected A extract. purification this CC led isolation pure which according its spectral data (6R,12aR)-2-amino-6-(1,3-benzodioxol-5-yl)-2,3,6,7,12,12a-hexahydropyrazino (1',2':1,6) pyrido (3,4-b) indole- 1,4-dione aminotadalafil. Conclusions. An inhibitor analogue, aminotadalafil, has supplement. This study first report Latin America one few independent studies an product ED treatment. Ulloa J, Sambrotta L, Redko F, Mazza ON, Garrido G, Becher EF, Muschietti L. Detection tadalafil analogue adulterant dysfunction. J Sex Med 2015;12:152-157.