Isolation and extraction of nuclei, chromosomes and chromosome components

摘要: Publisher Summary This chapter focuses on the isolation and extraction of nuclei, chromosomes, chromosome components. The primary requisite for nuclei is to isolate them in normal condition adequate amounts keep chromatin an undamaged state. Several factors control success isolation, which low temperature, medium, method homogenization, pH, divalent cations are most important should be suitably adjusted each tissue. procedure may direct or indirect. In former procedure, nucleus isolated directly from cytoplasm with aid a micromanipulator observed under microscope. procedures are, however, not free limitations. principal disadvantage lies methodology separation liquids needed suspension. Even weak solvents, there possibility decomposition sensitive compounds, adsorption cytoplasmic contents by nucleus, elimination diffusion some nuclear spite these limitations, techniques enable worker handle chromosomes laboratory, permitting correct analysis