Poly(l-lysine)-graft-dextran Copolymer Promotes Pyrimidine Motif Triplex DNA Formation at Physiological pH THERMODYNAMIC AND KINETIC STUDIES

摘要: Abstract Extreme instability of pyrimidine motif triplex DNA at physiological pH severely limits its use for artificial control gene expression in vivo. Stabilization the is therefore great importance improving therapeutic potential. To this end, isothermal titration calorimetry interaction analysis system and electrophoretic mobility shift assay have been used to explore thermodynamic kinetic effects our previously reported stabilizer, poly (l-lysine)-graft-dextran (PLL-g-Dex) copolymer, on formation pH. Both analyses clearly indicated that presence PLL-g-Dex binding constant was about 100 times higher than observed without any stabilizer. Of importance, triplex-promoting efficiency copolymer more 20 concentrations spermine, a putative intracellular Kinetic data also demonstrated copolymer-mediated promotion resulted from considerable increase association rate rather decrease dissociation constant. Our results certainly support idea could be key material may eventually lead progress applications antigene strategy