Identification and characterization of differentially expressed novel miRNAs (21–24 nt) in a Macrophomina phaseolina resistant RIL line of jute (Corchorus capsularis L.)

摘要: Abstract A RIL population of jute developed by crossing one resistant accession CIM 036 and a susceptible variety JRC 412 was used to identify novel defence related miRNAs activated upon challenged inoculation with stem rot pathogen Macrophomina phaseolina. About total 15.7 million reads were generated from the line read length 1 × 50 bp. Here, we identified nine mature microRNAs which passed Minimum Free Energy (MFE Kcal/mol) criteria. Target site secondary structure predicted most them showed ubiquitination selective autophagy activity high expression value. Five viz. Candidate_41, Candidate_9, Candidate_66, Candidate_65 Candidate_8 had free energy less than −25 kcal/mol. Known miR-845b miR-166 superfamily are abundantly expressed The sequence is identical that Arabidopsis thaliana except at 18th position, but unlike in A. thaliana it targets coding for P-loop motif mRNA sequences disease resistance proteins nucleotide binding (NBS) leucine-rich repeat (LRR) motifs. In-silico analysis suggested provided NBS-LRR ROS mediated subsequently enabled multi-layered cascade against M. phaseolina jute.