Molecular, proteomic and immunological parameters of allergens provide inclusion criteria for new candidates within established grass and tree homologous groups

摘要: Our knowledge of allergen structure and function continues to rise new scientific data on the homology cross-reactivity sources should be considered extend work Lorenz et al., 2009 (Int Arch Allergy Immunol. 148(1):1–1, 2009) concept homologous groups. In addition this, sophisticated techniques such as mass spectrometry (MS) are increasingly utilised better characterise complex mix nature extracts. Homology models were used Fag s 1 (Beech) Cyn d (Bermuda grass) compared with template crystal structures Bet v Phl p from ‘exemplar’ species Birch Timothy grass, respectively. ELISA experiments performed assess Beech (tree) Bermuda (grass) extracts rabbit sera raised either “3-Tree” (Birch, Alder Hazel) extract or “Grass” (12-grass extract), The comparability biochemical stability different was assessed through statistical methods for a range tree grass species. Allergen and/or structural have been described providing justification inclusion within group. Data (Cyn 1) provides further this into group sweet grasses. However, characterisation relevant allergens and, in particular, comparison cross-reactive patterns between subjects specifically areas high abundance both Pooideae Chloridoideae is sought. MS allows possibility identify individual proteins mixes by sequence, has extensively applied field. New homology, biological parameters context groups certainly dynamic allowing flexibility potential streamlining quality parameters, profiles, due extrapolation exemplar wider allergens.