Deletion mapping of chromosome 8p in prostate cancer by fluorescence in situ hybridization

摘要: Abstract Double-target fluorescence in situ hybridization (FISH) was applied to 42 cases of prostate cancer and seven histologically proven benign hyperplasia for the detection structural aberrations chromosome 8. Cosmid probes two 8p loci (LPL/8p22 D8S7/8p23) were used 34 specimens malignant tumors obtained by touch biopsy technique. Deletion defined as when number cosmid signals lower than centromere more 35% all nuclei observed. In total, thirty (71%) demonstrated any type deletion. Out which deletion mapping could be evaluated, distal (D8S7) detected 17 (50%), 10 also showed LPL. LPL 18 (53%), 8 (24%) retained D8S7 (interstitial deletion). When pattern graded (1) no (2) partial (either or deleted) (3) both deletions, degree well correlated with tumor grade (P = 0.0009) stage 0.0072, Fisher's Exact test). These data support hypothesis that suppressor gene(s) may located chromosomal region 8p22, hence deletions play a crucial role pathogenesis cancer.