L-argininamide improves the refolding more effectively than L-arginine

摘要: L-arginine (Arg) is a widely used additive for suppressing protein aggregation during refolding. Systematic screening of Arg analogs provides superior additives that enhance the refolding yield more effectively than Arg. The hen egg lysozyme in presence 500 mM L-argininamide (ArgAd) increases 1.7-fold higher Thermal unfolding experiments indicate ArgAd has greater denaturing effect positively relates to net charge analogs. Moreover was also effective bovine carbonic anhydrase. High potency increase compared results from high positive and property.