Ethanolic extracts of herbal supplement Equiguard suppress growth and control gene expression in CWR22Rv1 cells representing the transition of prostate cancer from androgen dependence to hormone refractory status.

摘要: Dietary supplements and botanical products are widely used by patients diagnosed with prostate cancer (CaP) as a primary or adjuvant form of treatment for their medical conditions in the United States. Many available complex mixtures composed extracts from foreign plants, whose mechanism action typically is not systematically rigorously investigated. Laboratory studies employing precisely defined referenced methodologies essential only standardization characterization products, but also important requisites providing scientific evidence molecular insights regard to clinical efficacies some these purportedly demonstrate. In previous this laboratory, we serendipitously observed that Equiguard, dietary supplement formulated nine Chinese herbs preventing decline renal functions associated aging process, contain 70% ethanol-extractable ingredients displayed potent growth inhibitory activities androgen-dependent (AD) LNCaP androgen-independent (AI) DU-145 PC-3 cells. Moreover, significant reduction expression androgen receptor (AR) specific antigen (PSA) occurred Equiguard-treated Although results offer possibility Equiguard confers chemoprevention CaP, it remains undetermined whether CaP cell types represent transition AD AI status. Further, details its have been fully elucidated. The described report focusing on CWR22Rv1 cells intended fill gaps. These express AR PSA, yet show weak responsiveness androgens largely proliferate an AI-independent manner - features mimic --> clinically advanced disease. Using cells, showed ethanolic effectively suppressed colony formation, inhibited proliferation, reduced cycle regulatory proteins including cyclin D1, E2F, well lowered PSA levels. Treatment decreased cyclooxygenase 2 led increases quinone reductase 1 2. provide further support possess multiple, chemopreventive attributes capable disrupting CaP.