NMR Studies of Nucleic Acids and Their Complexes

摘要: As the study of biological processes has progressed to molecular level understanding underlying mechanisms required determination three-dimensional structures molecules involved, their ability undergo structural interconversions, and interactions among them. For that form highly ordered crystals, X-ray diffraction provides very precise information. However, there are cases in which crystal packing affects structure, many for crystals have not been obtained. With development multidimensional experiments high-field spectrometers, NMR spectroscopy become an important alternative structure determination. obvious advantages needed, rather only moderately concentrated solutions, it is possible deal quite well with complications such as interconverting conformers. In addition, solution conditions can be varied widely, changing solvent composition, ions, pH, temperature so forth without worrying about disrupting crystallization conditions. These made attractive crystallography biomolecular (Wuthrich, 1986). still understand significant differences between two methods, should considered when interpreting data. usually true, best if results determined on same system by both these then compared.