Regulation of haptoglobin gene expression in 3T3-L1 adipocytes by cytokines, catecholamines, and PPARγ
作者： Claudia Oller do Nascimento , Leif Hunter , Paul Trayhurn
DOI: 10.1016/J.BBRC.2003.12.008
关键词:
摘要: Factors which regulate expression of the haptoglobin (acute phase reactant) gene in adipocytes have been examined using 3T3-L1 cells. Haptoglobin was observed by Northern blotting each major white adipose tissue depots mice (epididymal, subcutaneous, mesenteric, and perirenal) interscapular brown fat. Expression occurred mature adipocytes, but not stromal-vascular fraction. In cells, mRNA detected from day 4 after induction differentiation into adipocytes. Lipopolysaccharide cytokines, TNFalpha interleukin-6, resulted substantial increases adipocytes; increase (7-fold) highest with TNFalpha. Increases level were also induced dexamethasone, noradrenaline, isoprenaline, a beta3-adrenoceptor agonist. contrast, reduced nicotinic acid PPARgamma agonist, rosiglitazone. RT-PCR showed that expressed human (subcutaneous, omental). It is concluded stimulated inflammatory glucocorticoids, sympathetic system, while activation nuclear receptor strongly inhibitory.
elsevier.com
sci-hub.se 参考文章(34)
Said Lyoumi, Herve Puy, Fabienne Tamion, Catherine Bogard, Antony Leplingard, Michel Scotte, Roger Vranckx, Francis Gauthier, Martine Hiron, Maryvonne Daveau, Yves Nordmann, Jean Charles Deybach, Jean Pierre Lebreton, Heme and acute inflammation FEBS Journal. ,vol. 261, pp. 190- 196 ,(1999) , 10.1046/J.1432-1327.1999.00254.X
B. COUSIN, O. MUNOZ, M. ANDRE, A. M. FONTANILLES, C. DANI, J. L. COUSIN, P. LAHARRAGUE, L. CASTEILLA, L. PÉNICAUD, A role for preadipocytes as macrophage-like cells The FASEB Journal. ,vol. 13, pp. 305- 312 ,(1999) , 10.1096/FASEBJ.13.2.305
Andrzej Mackiewicz, Theodore Speroff, Mahrukh K Ganapathi, I Kushner, None, Effects of cytokine combinations on acute phase protein production in two human hepatoma cell lines. Journal of Immunology. ,vol. 146, pp. 3032- 3037 ,(1991)
S. Pajovic, V.E. Jones, K.R. Prowse, F.G. Berger, H. Baumann, Species-specific changes in regulatory elements of mouse haptoglobin genes Journal of Biological Chemistry. ,vol. 269, pp. 2215- 2224 ,(1994) , 10.1016/S0021-9258(17)42156-9
Claire M. Steppan, Shannon T. Bailey, Savitha Bhat, Elizabeth J. Brown, Ronadip R. Banerjee, Christopher M. Wright, Hiralben R. Patel, Rexford S. Ahima, Mitchell A. Lazar, The hormone resistin links obesity to diabetes Nature. ,vol. 409, pp. 307- 312 ,(2001) , 10.1038/35053000
P De Vos, A M Lefebvre, S G Miller, M Guerre-Millo, K Wong, R Saladin, L G Hamann, B Staels, M R Briggs, J Auwerx, Thiazolidinediones repress ob gene expression in rodents via activation of peroxisome proliferator-activated receptor gamma. Journal of Clinical Investigation. ,vol. 98, pp. 1004- 1009 ,(1996) , 10.1172/JCI118860
Fred Haugen, Aud Jørgensen, Christian A Drevon, Paul Trayhurn, Inhibition by insulin of resistin gene expression in 3T3-L1 adipocytes. FEBS Letters. ,vol. 507, pp. 105- 108 ,(2001) , 10.1016/S0014-5793(01)02968-4
M. Arredouani, P. Matthijs, E. Van Hoeyveld, A. Kasran, H. Baumann, J. L. Ceuppens, E. Stevens, Haptoglobin directly affects T cells and suppresses T helper cell type 2 cytokine release Immunology. ,vol. 108, pp. 144- 151 ,(2003) , 10.1046/J.1365-2567.2003.01569.X
P. Trayhurn, J. Duncan, A. Wood, J. Beattie, Regulation of metallothionein gene expression and secretion in rat adipocytes differentiated from preadipocytes in primary culture. Hormone and Metabolic Research. ,vol. 32, pp. 542- 547 ,(2000) , 10.1055/S-2007-978683
Peter C. Scriba, Manfred Bauer, Dieter Emmert, Ahmed Fateh-Moghadam, Gert G. Hofmann, Klaus Horn, C. Renate Pickardt, EFFECTS OF OBESITY, TOTAL FASTING AND RE-ALIMENTATION ON l-THYROXINE (T4), 3,5,3'-l-TRIIODOTHYRONINE (T3), 3,3',5'-l-TRIIODOTHYRONINE (rT3), THYROXINE BINDING GLOBULIN (TBG), CORTISOL, THYROTROPHIN, CORTISOL BINDING GLOBULIN (CBG), TRANSFERRIN, α2-HAPTOGLOBIN AND COMPLEMENT C'3 IN SERUM European Journal of Endocrinology. ,vol. 91, pp. 629- 643 ,(1979) , 10.1530/ACTA.0.0910629