The measurement of lipid peroxidation in isolated hepatocytes
作者： Martyn T. Smith , Hjördis Thor , Pia Hartzell , Sten Orrenius
DOI: 10.1016/0006-2952(82)90230-1
关键词:
摘要: Different techniques for the measurement of lipid peroxidation in isolated hepatocytes have been compared. Measurements ethane production, chemiluminescence and fluorescent products correlated extremely well with those malondialdehyde formation. Of five different studied, measurements production were found to be most sensitive indices peroxidation. Incubation up 4 hr presence ethylmorphine aminopyrine, at concentrations known stimulate H202 completely failed increase amount chemiluminescence, or produced these cells, indicating that drug-stimulated H2O2 did not lead an increased rate liver cells under experimental conditions employed. The relationship between peroxidation, as measured by cytotoxic effects bromobenzene carbon tetrachloride has also studied. results obtained further indicate is important event hepatotoxicity, but it appears only a subsequent toxicity, possibly occurring result ofglutathione depletion cell death.
elsevier.com
sci-hub.se 参考文章(33)
Frederick Bernheim, Mary L.C. Bernheim, Karl M. Wilbur, The reaction between thiobarbituric acid and the oxidation products of certain lipides. Journal of Biological Chemistry. ,vol. 174, pp. 257- 264 ,(1948) , 10.1016/S0021-9258(18)57394-4
Michael A. Trush, Mark E. Wilson, Knox van Dyke, [39] The generation of chemiluminescence (CL) by phagocytic cells Methods in Enzymology. ,vol. 57, pp. 462- 494 ,(1978) , 10.1016/0076-6879(78)57041-9
H E May, P B McCay, Reduced Triphosphopyridine Nucleotide Oxidase-catalyzed Alterations of Membrane Phospholipids II. ENZYMIC PROPERTIES AND STOICHIOMETRY Journal of Biological Chemistry. ,vol. 243, pp. 2296- 2305 ,(1968) , 10.1016/S0021-9258(18)93475-7
S Orrenius, D P Jones, H Thor, B Andersson, Detoxification reactions in isolated hepatocytes. Role of glutathione peroxidase, catalase, and formaldehyde dehydrogenase in reactions relating to N-demethylation by the cytochrome P-450 system. Journal of Biological Chemistry. ,vol. 253, pp. 6031- 6037 ,(1978) , 10.1016/S0021-9258(17)34575-1
ED Wills, AE Wilkinson, Release of enzymes from lysosomes by irradiation and the relation of lipid peroxide formation to enzyme release. Biochemical Journal. ,vol. 99, pp. 657- 666 ,(1966) , 10.1042/BJ0990657
Armun Müller, Peter Graf, Albrecht Wendel, Helmut Sies, Ethane production of isolated perfused rat liver: A system to study metabolic effects related to lipid peroxidation FEBS Letters. ,vol. 126, pp. 241- 244 ,(1981) , 10.1016/0014-5793(81)80251-7
Peter Moldéus, Johan Högberg, Sten Orrenius, [4] Isolation and use of liver cells Methods in Enzymology. ,vol. 52, pp. 60- 71 ,(1978) , 10.1016/S0076-6879(78)52006-5
Catherine T. Hammer, Eric D. Wills, The role of lipid components of the diet in the regulation of the fatty acid composition of the rat liver endoplasmic reticulum and lipid peroxidation Biochemical Journal. ,vol. 174, pp. 585- 593 ,(1978) , 10.1042/BJ1740585
E. D. Wills, Lipid peroxide formation in microsomes. General considerations Biochemical Journal. ,vol. 113, pp. 315- 324 ,(1969) , 10.1042/BJ1130315
Christian Steffen, Hassan Muliawan, Hermann Kappus, Lack of in vivo lipid peroxidation in experimental paraquat poisoning. Naunyn-schmiedebergs Archives of Pharmacology. ,vol. 310, pp. 241- 243 ,(1980) , 10.1007/BF00499917