Hamycin inhibits IL-8-induced biologic response by modulating its receptor in human polymorphonuclear neutrophils.

摘要: IL-8, a neutrophil chemotactic agent, is involved in large number of neutrophil-driven acute and chronic inflammatory diseases. We have found that hamycin, an antifungal reduces IL-8-induced migration binding 125I-labeled IL-8 to neutrophils by 66 75%, respectively. Other biologic functions, such as superoxide generation, intracellular Ca2+ mobilization, enzyme release were also reduced hamycin-treated cells 50 75%. Anti-IL-8R Ab (C-X-CR1) itself failed protect the from effect hamycin. Scatchard analysis data demonstrated while normal expressed 23,000 +/- 1,704 receptors/cell (Kd = 3.5 nM), was 8,000 592 3.43 nM) cells. Chemical cross-linking its receptor followed 10% SDS-PAGE autoradiography showed signals considerably compared with those controls. In immunoblot, however, control almost identical. The intensity fluorescence emission diphenyl hexatriene at 430 nm membrane microviscosity measured cells, resulting functional IL-8R, presumably conformational change receptor. study suggests hamycin may be potent immunomodulator IL-8R for alleviation distress.