Zebrafish sperm cryopreservation.

摘要: Publisher Summary This chapter reviews the important elements that should be considered when developing a sperm cryopreservation protocol in zebrafish. It also presents an alternative freezing method and discusses further considerations future directions for continued optimization of zebrafish cryopreservation. The goal is to provide safe chemical environment reducing cellular temperatures below –130 °C indefinitely store cells inactive state able thaw recover these without compromising physiological function. Cryopreservation from teleosts such as additional complication they must not prematurely activated by water exposure, which needed postthaw, trigger changes intracellular potassium concentration required motility fertilization. An optimal technique protect spermatozoa damage while maintaining inactivity. high variability effective cryoprotectants within across species emphasizes need evaluate many cryoprotectant combinations determining medium Two methods are used collect sperm: abdominal massage homogenization testes removed dissection. provides conditions frozen any healthy male consistently offspring through vitro In addition its higher fertilization rate, this yields four cryopreserved samples per individual. increases potential recovery rate fourfold can extend period time recovering embryos given line, helping preserve genetic diversity.